HYDROLYTIC ENZYMES OF RABBIT MONONUCLEAR EXUDATE CELLS

Hydrolytic Enzymes of Rabbit Mononuclear Exudate Cells

Oil-induced mononuclear phagocytes (MN) were quantitatively assayed for various hydrolases as unfractionated suspensions of frozen and thawed cells. They apparently contain two proteases. The first, measured with urea- or acid-denatured hemoglobin, was similar to purified Proteinase I of lung with respect to pH optimum (pH 4), stability, hydrolytic and polymerizing activities, and reactions to ...

Hydrolytic Enzymes of Rabbit Mononuclear Exudate Cells. Ii. Lysozyme: Properties and Quantitative Assay in Tuberculous and Control Inbred Rabbits.

HYDROLYTIC ENZYMES OF RABBIT MONONUCLEAR EXUDATE CELLS I. Quantitative Assay and Properties of Certain Proteases, Non-Specific Esterases, and Lipases of Mononuclear and Polymorphonuclear Cells and Erythrocytes

Oil-induccd mononuclear phagocytes (MN) were quantitatively assayed for various hydrolases as unfractionatcd suspensions of frozen and thawed cells. They apparently contain two proteases. The first, measured with ureaor acid-denatured hemoglobin, was similar to purified Proteinase I of lung with respect to pH optimum (pH 4), stability, hydrolytic and polymerizing activities, and reactions to va...

A Histochemical Study of Phagocytic and Enzymatic Functions of Rabbit Mononuclear and Polymorphonuclear Exudate Cells and Alveolar Macrophages

The cytochrome oxidase (CO), aminopeptidase (AMP), succinic dehydrogenase (SD), acid phosphatase, esterase, and alkaline phosphatase of rabbit mononuclear (MN) and polymorphonuclear (PMN) peritoneal exudate cells and pulmonary alveolar macrophages (AM) - air dried on Mylar strips - were characterized by histochemical techniques with respect to stability, activators, inhibitors, and pH optima. A...

Hydrolytic enzymes of Moraxella bovis.

Certain strains of Moraxella bovis produce tissue-damaging enzymes which may initiate or potentiate infectious bovine keratoconjunctivitis. Thirteen reference strains of this species were characterized physiologically and screened for production of various enzymes by some conventional biochemical tests and the API ZYM system (Analytab Products, Plainview, N.Y.). All 13 strains were hemolytic. A...